abstract in this experiment the effects of two type of medium cultures, gibberellic acid and dark/cold and light/warm culture conditions on mature embryos germination of a local cultivar of walnut were investigated. the mature fruits were collected at 22 weeks postanthesis and after disinfection the mature embryo along with cotyledon pieces were excised and then were cultured on mt and modified...

the present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of fritillaria imperialis l. there is no published method recommended for protoplast isolation and regeneration from fritillaria imperialis l. a range of factors, which influence the success of isolation and regeneration of f. imperialis protoplasts, were investigated. from the results obta...

background and objectives : there are limitations in time and technique for isolation of brucella from patients. we developed a new brucella culture medium and evaluated its efficiency compared to bactec blood culture system and serology. materials and methods: a bi-phasic medium containing urea agar and brain heart infusion was formulated. appearance of clear red color in liquid phase was the ...

the major objective of seedless grape breeding program is production of grapes with high quality. breeding of seedless grapes is difficult because the embryos are aborted after fertilization. embryo rescue technique is utilized to obtain plants from crosses between seedless cultivars. in the present study, the effect of paternal genotypes and different iaa concentrations in presence of ba appli...

background: in the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. a smaller medium drop can prevent dilution of this factor(s). objective: the objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. materials and methods: the em...

the germination power of orchids (orchidaceae family) seems to betoo weak due lack of albumen. the study carried out with various treatments including pollination time and ga3 for breaking dormancy and increasing seed germination of orchids. the effect of pollination time (8 periods from january to august) and gibberellic acid (0, 500, 1000 and 1500 mg l-1) were studied on germination of phalae...

A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involved the introduction of blood (and bacteria) into the growth medium with the dye...

for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...